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Proteinase K CAS 39450-01-6 

Purity: ≥95% (Native-PAGE)

Appearance: white lyophilized powder

Packaging: 10g; 20g; 50g; 100g/bottle;

Sample: available

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Description

Proteinase K Quick Details

Chemical Name: Proteinase K

CAS No.: 39450-01-6

Chemical structure:

Molecular Formula:C29H27N2O12P

EINECS: 254-457-8

Synonyms:Endopeptidase K;Proteinase K;Tritirachium album proteinase K;

Appearance: white lyophilized powder

Proteinase K Typical Properties

Item Standard
Appearance white lyophilized powder
Purity ≥95% (Native-PAGE)
Specific activity value Dry powder 40 U/mg protein, containing 50% lyoprotectant
Dilution buffer 20 mM Tris-Cl, 1mM CaCl2, pH 7.4,50% Glycerol.
Derived from Tritirachium album limber
Residue of deoxyribonuclease using λDNA as substrate, digest at 37 ℃ for more than 6 hours, no deoxyribonuclease activity was detected
Residue of ribonuclease digestion at 25℃for more than 16 hours, no ribonuclease activity was detected.

Proteinase K Application

Proteinase K is stable in urea and SDS, and also has the ability to degrade natural proteins, so it is widely used, mainly used in gene diagnostic kits, genomic DNA extraction kits, RNA extraction kits to remove DNA and RNA preparation nucleic acids Enzymes can also be used to extract non-protein components in tissues to degrade protein-containing impurities. For example, the preparation of DNA vaccines and heparin.

Proteinase K Packaging 

10g; 20g; 50g; 100g/bottle;

Proteinase K Storage

The dry powder state can be stored at low temperature under the condition of 0~4℃. After dissolving, dispense into appropriate volume and store at -15℃-25℃. Transport can be carried out at room temperature.
Under the correct storage conditions, the dry powder state is valid for up to two years. The validity period of the liquid state at -15~25℃ is one year. After opening the package, please use it in time to prevent moisture absorption. After the solid enzyme is dissolved, it is recommended to filter and sterilize, divide it into an appropriate volume, and store at -15~25℃.